FIGURE 1.

Generation of transgenic silkworms that express H1/FGF‐7 in the posterior silk gland. A) Schematic representation of the plasmid vector used to generate transgenic silkworms. The plasmid vector pBacMCS[UAS‐H1/FGF‐7‐SV40/3xP3‐EGFP] encoding H1/FGF‐7 was used to generate the transgenic line termed UAS‐H1/FGF‐7. The piggyBac right and left inverted terminal repeats (ITRs) (L and R) are indicated by arrows. B) Generation of the FH‐H1/FGF‐7 line. The FH‐GAL4 line ²⁹ was mated with the UAS‐H1/FGF‐7 line to generate the FH‐H1/FGF‐7 line in which expression of EGFP and discosome sp. Red (DsRed) as genetic markers is visible in the eyes. C) Generation of the FH‐poly/H1/FGF‐7 line. The FH‐polyhedrin line was mated with the UAS‐H1/FGF‐7 line to generate the FH‐poly/H1/FGF‐7 line with the expression of EGFP and DsRed visible in the eyes and kynurenine 3‐monooxygenase (KMO) specific expression in the skin, ⁷ as genetic markers.