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. 2020 Oct 20;2(12):734–744. doi: 10.1096/fba.2020-00078

FIGURE 3.

FIGURE 3

Detection of H1/FGF‐7 associated with polyhedra. A, Detection of H1/FGF‐7 in polyhedra using immunoblotting. Polyhedra (50,000 cubes) collected from PSGs of FH‐polyhedrin (lane 1, ref. 7) and FH‐poly/H1/FGF‐7 larvae (lane 2) were analyzed using immunoblotting with anti‐FGF‐7 antibodies. B, Confocal microscopy analysis of H1/FGF‐7 and polyhedrin expression on polyhedra by immunofluorescence. Polyhedra collected from PSGs of FH‐poly/H1/FGF‐7 larvae were fixed on a glass‐base dish and examined using immunofluorescence with anti‐FGF‐7 antibody and anti‐polyhedrin antibody. Scale bars, 10 µm