FIGURE 6.

3D keratinocyte cultures. A, Hematoxylin and eosin (HE) staining in a 3D culture of keratinocytes. NHEKs were cultured on a collagen gel containing 800 µg posterior silk gland powders (PSGPs) from FH‐polyhedrin or FH‐poly/H1/FGF‐7 larvae in the basal medium for 2 days and in a differentiation medium (basal medium containing 1.2 mM Ca²⁺) for 14 days. The NHEKs were cultured in the same way on collagen with 100 ng/ml rhFGF‐7 in basal and differentiation media as a control. After cultivation for 14 days in differentiation media, 3D cultures were sectioned, subjected to HE staining and observed under a microscope. Scale bar, 50 µm. B, Immunohistochemical staining of keratinocyte 3D cultures. Immunohistochemical staining of keratin 10 (K10), keratin 14 (K14), Loricrin (Lor), and Filaggrin (Fil) in 3D cultures of keratinocytes incubated with PSGPs from FH‐poly/H1/FGF‐7 larvae. Nuclei were stained with 4′,6‐diamidino‐2‐phenylindole. Scale bar, 50 µm