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. Author manuscript; available in PMC: 2020 Dec 14.
Published in final edited form as: Cell Rep. 2016 Sep 27;17(1):275–288. doi: 10.1016/j.celrep.2016.09.003

Figure 2. Cytokine genes are aberrantly up-regulated in ARID1A/PIK3CA mutant cells.

Figure 2.

(A) Heat-map of the genes commonly up-regulated in T29 (AM) and T80 (AM) cells compared to all other cell lines (FC>=2).

(B) Genes identified in panel (A) are enriched in the up-regulated genes in OCCC. Genes up-regulated in patient OCCC samples relative to normal tissues (from GSE6008) were ranked from left to right, and gene set enrichment on the up-regulated genes can be seen on the top and middle panels.

(C) qRT-PCR verification of the RNA-Seq result. mRNA level of selected cytokines genes were validated by qRT-PCR, normalized by β-actin transcripts (n=3 for all experiments except CXCL1 in T29, of which n=4). Error bars, S.E.M.

(D) IL-6 antibody can partly block T80 (AM), but not T80, cell growth. Control T80 or T80 (AM) cells were incubated with anti-mouse IgG or IL-6 neutralizing antibody (2 ng/ml) (n=3) and the cell proliferation rate was measured. Error bars, S.E.M.

*, p-value < 0.05; **, p-value < 0.01. See also Figure S2 and Supplemental Table S1.