Supplemental Figure S3.

Changes in lung lymphatics after bleomycin administration and in type I collagen after telomere dysfunction. A: Abundance of lung lymphatics (area density, expressed as fold difference from baseline) from onset to 56 days after bleomycin administration. The greatest relative change in lymphatics was in the lung parenchyma, because of the small number at baseline. Significantly different from baseline. Dotted line indicates zero on y axis. B–G: Changes in α-smooth muscle actin and type I collagen immunoreactivity in mice with telomere dysfunction. Lungs of wild-type and telomere-deficient, CCSP-Cre/TRF1-floxed mice and SPC-Cre/TRF1-floxed mice at 9 months of tamoxifen. B–G: Stained for vascular endothelial growth factor receptor (VEGFR)-3 (lymphatics) and α-smooth muscle actin (B–D) or type I collagen (E–G). Amounts of α-smooth muscle actin and type I collagen is greater in mice with telomere dysfunction. n = 4 to 8 mice per group (A). ∗P < 0.05 versus baseline (untreated) lungs (analysis of variance). Scale bar = 200 μm (B–G).