Supplemental Figure S6.

A: Validation of allograft inflammatory factor 1/ionized calcium binding adapter molecule 1 (Aif1/Iba1) as a marker of interstitial macrophages. Aif1/Iba1 largely colocalized with Cx3cr1 and mannose receptor Mrc1/Cd206 in a baseline Cx3cr1-enhanced green fluorescent protein (EGFP) mouse lung. Pleural surface is at top of image. All three antibodies stain a largely overlapping, but not identical, population of macrophages. Cx3cr1-EGFP marks the largest number of cells, including small globular cells that are likely monocytes. Mrc1/Cd206 also stains large globular cells, likely alveolar macrophages, and linear or tubular structures stained. B: These are identified as initial lymphatics in a baseline prospero homeobox 1 (Prox1-EGFP) lung. Scale bar = 50 μm (A and B).