Supplemental Figure S8.

Lung lymphatic growth after vascular endothelial growth factor (Vegf) c overexpression in CCSP/VEGF-C mice. CCSP-rtTA/TetO-VEGF-C double-transgenic and single-transgenic controls received doxycycline (1 mg/mL in drinking water) at weaning from postnatal (P28) to P35. A and B: Low-magnification panoramas of left lung of single-transgenic control (A) and double-transgenic mouse with lymphatic network expansion (B). Lymphatics are most abundant around large airways that have VEGF-C–overexpressing club cells. C: Dot plot showing the distribution of the increase in lymphatics (area density) in the lung hilum of CCSP/VEGF-C mice. Significantly different from single-transgenic controls. n = 4 mice per group (C). ∗P < 0.05 versus control (analysis of variance). Scale bar = 1 mm (A and B).