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. 2020 Dec;190(12):2355–2375. doi: 10.1016/j.ajpath.2020.08.018

Figure 2.

Figure 2

Lung lymphatics: Apoptosis during recovery after bleomycin treatment and network expansion with telomere dysfunction. A: Activated caspase-3 staining in and around lung lymphatics stained for prospero homeobox 1-enhanced green fluorescent protein (Prox1-EGFP) and vascular endothelial growth factor receptor (VEGFR)-3 at 56 days after bleomycin administration. B–D: Low-magnification panoramas of sections (200 μm thick) of left lungs of 9-month–old mice with telomere dysfunction stained for lymphatics (green) and smooth muscle (red). B: Baseline [wild-type (WT) littermate]. C: CCSP-Cre/TRF1-floxed mouse. D: SPC-Cre/TRF1-floxed mouse. E–G: Abundance of lymphatics (area density) overall and in hilum and parenchyma in baseline mice, CCSP-Cre/TRF1 mice, and SPC-Cre/TRF1 mice. Dots represent individual mice. n = 3 to 4 mice per group (E–G). ∗P < 0.05 versus baseline (analysis of variance). Scale bars: 50 μm (A); 1 mm (B–D).