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. 2020 Dec;190(12):2355–2375. doi: 10.1016/j.ajpath.2020.08.018

Figure 5.

Figure 5

Vascular endothelial growth factor receptor (Vegfr) 3 dependency of lymphatic growth and chemokine (C-C motif) ligand (Ccl) 21 expression but not of bronchoalveolar lavage fluid (BALF) indexes of lung inflammation. A and B: Expression of Prox1 and Vegfr3 (quantitative RT-PCR) in lungs at baseline and 14 days after bleomycin administration. C and D: CCL21 immunoreactivity in lungs at 14 days after bleomycin challenge, showing large increase in staining when accompanied by control antibody (IgG) but suppression of lymphatic growth and CCL21 staining when accompanied by mF4-31C1 (anti-Vegfr3). E–G: Lack of difference in BAL protein (E), leukocytes (F), and bronchial lymph node weight (G) of mice given bleomycin with control IgG or mF4-31C1 (anti-Vegfr3). Dots represent individual mice. Blue dots, baseline (no bleomycin); black dots, 14-day bleomycin + IgG; green dots, 14-day bleomycin + mF4-31C1 anti-Vegfr3 antibody. H: Body weights of same groups of mice are significantly different from one another (P < 0.0025, Kolmogorov-Smirnov test). Dots represent means of groups. n = 4 to 20 mice per group (E–G); n = 20 no bleomycin (H); n = 7 bleomycin + mF4-31C1 (H); n = 9 bleomycin + IgG (H). ∗P < 0.05 versus baseline (t-test); †P < 0.05 versus baseline (analysis of variance). Scale bars = 200 μm (C and D). Prox1-EGFP, prospero homeobox 1-enhanced green fluorescent protein; WBC, white blood cell.