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Size exclusion chromatography. A) chromatogram showing the eluted peak of the size-exclusion column. The asymmetrical peak appears to contain two species, dimer (85 mL) and monomer (92.5 mL), of MVpro. B) 15% SDS-PAGE gels stained with Coomassie Brilliant Blue showing the eluted fractions of pure MVpro. Two bands are apparent: ~20 kDa, and ~ 19 kDa which suggests auto-proteolysis of the C-terminus.
