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. 2020 Dec 14;18:483. doi: 10.1186/s12967-020-02657-6

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Combined treatment with MDSCs and Treg control alloreactive T cell response. a In the mixed lymphocyte reaction assay, a total of 1 × 10⁵ B/c splenic T cells (responders) were incubated with 1 × 10⁵ irradiated B/c (syngeneic stimulators, Syn) or B6 (allogeneic stimulators, Allo]) splenic APCs for 4 days. Responder cells were cultured in the presence or absence of MDSC (2 × 10⁴) or/and Treg (2 × 10⁴). b IFN-γ and IL-17 levels in the supernatants were measured by ELISA. c Intracellular immunostaining of IL-17 + CD4 + , IFN-γ + CD4 + , Foxp3 + Treg cells was performed were determined by flow cytometry. Data are means ± SEMs. Data are representative of three independent experiments (*p < 0.05, **p < 0.01, ***p < 0.001 versus alloresponse)