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. Author manuscript; available in PMC: 2021 Aug 4.
Published in final edited form as: Cell Metab. 2020 Jul 7;32(2):273–286.e6. doi: 10.1016/j.cmet.2020.06.008

Figure 3. The role of FGF21 signaling to the PVN.

Figure 3.

(A-C) Fluid intake during two-bottle choice of 10% sucrose versus water in in 11–13 week old male WT mice or mice lacking KLB in (A) Oxytocin- (KLB Oxy-KO), (B) arginine vasopressin-(KLB AVP-KO), or (C) Sim1-expressing cells (KLB Sim1-KO) treated with vehicle or FGF21 (1 mg/kg) i.p. injection for 3 days (n = 6–15/group).

(D and E) Immunofluorescence imaging for cFos in the (D) paraventricular nucleus of the hypothalamus (PVN) or (E) ventromedial hypothalamus (VMH) from Klb-CRE;Ai14 tdTomato mice treated with vehicle or FGF21 (1 mg/kg) i.p. injection for 1 hour (white arrows, cFos and KLB double-positive cells).

Values are mean +/− SEM. (*, P < 0.05 compared to WT). Statistical analyses were conducted using 2-way ANOVA for multiple comparisons.