Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Dec 14;21:891. doi: 10.1186/s12864-020-07306-2

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 1 — PEDV infection in IPEC-J2 cells. a Fluorescence-based cytotoxicity assay (using the LIVE/DEAD Viability/Cytotoxicity kit) of IPEC-J2 cells treated with PEDV strain CV777 at different time points. The green color indicates live cells stained by Calcein-AM and the red color indicates dead cells stained by propidium iodide. The green color was predominant in all viability assays, with only a few red (dead) cells appearing randomly. A significantly higher percentage of apoptotic cells (live (green)/dead (red)) are observed in the infected groups than in the controls (magnification, ×40; bars, 200 μm). b The mRNA expression level of MX1 gene in PEDV-infected IPEC-J2 cells as measured by qRT-PCR at different time points. c The mRNA expression level of M gene in PEDV-infected IPEC-J2 cells as measured by qRT-PCR at different time points. Data derive from three independent experiments and were analyzed by one-way ANOVA. Data are presented as the mean ± SD (n = 6). *** represents p < 0.001