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. 2020 Dec 14;21:891. doi: 10.1186/s12864-020-07306-2

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Identification of differentially expressed genes. a Volcano plots of differentially expressed genes between the mock-infected and PEDV-infected groups. The red and green dots represent upregulated and downregulated genes, respectively, in the PEDV-infected group compared with the mock-infected group. b Heatmap showing the expression levels of the differentially expressed genes. Columns represent individual samples and rows indicate genes with significant expression differences between the two groups. c qRT-PCR validation of the differentially expressed genes. The left axis represents gene expression levels as determined by qRT-PCR, and the right axis represents the expression levels determined by RNA-seq in FPKM units. mRNA expression levels were normalized to the mRNA levels of the pig ACTB gene. Graphed data represent the mean ± SD, n = 6. * represents p < 0.05, ** represents p < 0.01, *** represents p < 0.001