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. 2020 Nov 30;8:543807. doi: 10.3389/fbioe.2020.543807

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Copyright © 2020 Pang, Shin and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of RuBisCO activase (Rca) co-expression and introduction of Pdc-based pathway (designated as Pdc) on (A) total metabolite yields and (B) glucose consumption and (C) carbon recovery in Escherichia coli during in situ CO₂ recycling. Errors represent standard deviation with n = 3. Abbreviation: rbcLS represents form I RuBisCO originated from Synechococcus PCC6301; AfcbbLS and AfcbbM are form I and form II RuBisCOs originated from A. ferrooxidans ATCC 23270. Rca1 and Rca2 are type I and II RuBisCO activases originated from A. ferrooxidans ATCC 23270. Pdc represents the Pdc-based pathway consisting of pyruvate decarboxylase (Pdc) and alcohol dehydrogenase (AdhB).