TABLE 1.
List of bacteria strains and plasmids used in this study.
| Name | Description | Ref. |
| Bacterial strains | ||
| E. coli BL21 (DE3) | F–, dcm, ompT, gal, lon, hsdSB(rB–, mB–), λ(DE3[lacI, lacUV5-T7 gene 1, ind1, sam7,nin5]) | Lab stock |
| E. coli DH5α | F– endA1 glnV44 thi-1 recA1 relA1 gyrA96 deoR nupG purB20 φ80dlacZΔM15 Δ(lacZYA-argF)U169, hsdR17(rK–mK+), λ– | Lab stock |
| E. coli MZLF | E. coli BL21(DE3) Δzwf ::FRT ldh ::FRT frd::FRT | Yang et al., 2016 |
| E. coli MZLFA | MZLF harboring pBAD-his6-prkA-pACYC184 and rbcLS-pET30a(+) | This study |
| Plasmids | ||
| pBAD-his6-prkA-pACYC184 | Recombinant plasmid carries prkA gene (derived from Synechococcus PCC7942) for the overexpression of phosphoribulokinase (PrkA) under the control of PBAD. | Parikh et al., 2006 |
| rbcLS-pET30a(+) | Recombinant plasmid carries rbcLS gene (originated from Synechococcus PCC6301) for the overexpression of engineered RuBisCO under the control of PT7 | Parikh et al., 2006 |
| pLOI295 | Recombinant plasmid carries pdc and adhB genes (derived from Zymomonas mobilis) for overexpression of Pdc-based carbon tap valve under the control of Plac | Hespell et al., 1996 |
| pET30b AfcbbLS | Recombinant plasmid carries cbbLS gene (originated from A. ferrooxidans ATCC 23270) for overexpression of RuBisCO under the control of P7. | Tsai et al., 2015 |
| pAfcbbM | Recombinant plasmid carries cbbM gene (AFE_2155) for overexpression of RuBisCO under the control of P7. | This study |
| pAfcbbQ1 | The RuBisCO activase gene cbbQ1 (originated from A. ferrooxidans ATCC 23270) fused with the His6-ubiquitin fragment at the native N termini, was amplified from pHueAfcbbQ1 and cloned into the vector pCDFDuet-1 with NcoI/HindIII sites. | This study |
| pAfcbbQ2 | The RuBisCO activase gene cbbQ2 (originated from A. ferrooxidans ATCC 23270) fused with the His6-ubiquitin fragment at the native N termini, was amplified from pHueAfcbbQ2 and cloned into the vector pCDFDuet-1 with NcoI/HindIII sites. | This study |
| pAfRca1 | The gene cbbO1 (AFE_3054) was amplified from pBAD33UbAfcbbO1 and cloned into pAfcbbQ1 vector with AsisI-XhoI | This study |
| pAfRca2 | The gene cbbO2 (AFE_2157) was amplified from pBAD33UbAfcbbO2 and cloned into pAfcbbQ2 with AsisI-XhoI | This study |