TABLE 2.

Specific activities of RuBisCO with RuBisCO activase (Rca) in E. coli BL21(DE3) crude lysate^a.

Sampleb	Specific Activity of RuBisCO in crude lysatec
	μmol mg–1 min–1
rbcLS	0.3 ± 0.2
AfcbbLS	0.5 ± 0.1
AfcbbM	0.5 ± 0.2
rbcLS+ Rca1	1.2 ± 0.3
AfcbbLS+ Rca1	2.2 ± 0.5
AfcbbM+ Rca2	2.6 ± 0.5

^a IPTG was supplemented (final concentration = 0.02 mM) for RuBisCO and RuBisCO activase (Rca) expression when OD₆₀₀ of bacterial cultures reached 0.4. RuBisCO and Rca were separately expressed in E. coli BL21(DE3). Protein quantification was carried out using the Bradford method.

^b rbcLS represents form I RuBisCO originated from Synechococcus PCC6301. AfcbbLS and AfcbbM are form I and form II RuBisCOs originated from A. ferrooxidans ATCC 23270. Rca1 and Rca2 are type I and II RuBisCO activases originated from A. ferrooxidans ATCC 23270.

^c Errors represent standard deviation with n = 3.