Figure 1.

S. Typhimurium Rck and Y. enterocolitica Invasin are able to induce both adhesion and invasion in intestinal epithelial cells. HCT116 cells were infected with MC-GFP, MC-RckGFP or MC-InvGFP strain for 1 h at 37°C (MOI 10:1). The percentage of total cell-associated (A) and intracellular (B) bacteria were calculated as the ratio of CFU of cell lysates and inoculum as described in Materials and Methods. Results are mean ± SD of results obtained from three independent experiments with two infected wells per experiment. Data were compared using a Mann-Whitney test (**p < 0.01; ****p < 0.0001).