FIGURE 4.

Model of aerobic and anaerobic metabolism of hydrogen connected with carbon dioxide assimilation in At. ferrooxidans. Solid green arrows indicate direct electron transfer, solid orange arrows indicate reverse electron transfer, and solid gray arrows indicate enzymatic reactions during the aerobic and anaerobic metabolism. Solid blue arrows indicate direct electron transfer during aerobic metabolism, while solid red arrows indicate direct electron transfer during anaerobic metabolism. The thickness of the colored arrows corresponds to the pathway significance according to the relative protein abundance in Figure 3. Dotted gray arrows indicate proton transfer, dotted green arrows indicate hydrogen influx, and dotted orange arrows indicate carbon dioxide influx. The color of proteins and multiprotein complexes corresponds to the log₂ fold changes in Figure 2. The redox potential values (E_H) for individual proteins and multiprotein complexes are referenced in the text. 2-PG, 2-phosphoglycolate; 3-PGA, 3-phosphoglycerate; 1,3-BPG, 1,3-bisphosphoglycerate; G3P, glyceraldehyde 3-phosphate; DHAP, dihydroxyacetone phosphate; SBP, sedoheptulose 1,7-bisphosphate; S7P, sedoheptulose 7-phosphate; E4P, erythrose 4-phosphate; FBP, fructose 1,6-bisphosphate; F6P, fructose 6-phosphate; R5P, ribose 5-phosphate; X5P, xylulose 5-phosphate; Ru5P, ribulose 5-phosphate; RuBP, ribulose 1,5-bisphosphate; PEP, 2-phosphoenolpyruvate; TCA, tricarboxylic acid.