Figure 1.

Transcript counts (log base 2 transformed) of the two target genes, (a) hsp70 and (b) hsp90, and the two putative reference genes, (c) gapdh and (d) actin, at the two temperature treatments (A = ambient, 19°C; H = high, 25°C). Lines show the log₂ transcript counts for each individual male. Points show Bayesian posterior means and bars show 95% highest probability density intervals, calculated using an ‘informed’ model in the package ‘MCMC.qpcr’ ([66]; see Methods), with actin specified as a reference gene (results were qualitatively the same for a ‘naive’ model without any reference genes specified; see Results). Estimates for actin itself are from the naive model. (Online version in colour.)