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. 2020 Dec 14;20:596. doi: 10.1186/s12935-020-01692-z

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — The effects of GPX8 on the Wnt signaling pathway. a, b The expression levels of key proteins (wnt1, wnt3a, p-GSK3β, GSK3β and β-catenin) of the Wnt signaling pathway were examined using western blot in normal human gastric epithelial cells (GES-1) and Gastric cancer cell lines (NCI-N87, SNU-16, SNU-5 and HGC-27). c, d After HGC-27 cells were transfected with blank control vector, pcDNA-control, pcDNA-GPX8, si-NC and si-GPX8, the expression levels of wnt1, wnt3a, p-GSK3β, GSK3β and β-catenin were detected using western blot. After pcDNA-GPX8 combined with or without Wnt inhibitor was co-transfected into HGC-27 cells, c the cellular proliferation was assessed by CCK-8 assay; f Colony formation assay, transwell migration and invasion assays were utilized to respectively detect the cellular colony formation, migration and invasion abilities. The images of data quantification of g colony formation, h transwell migration and i migration assays. ****p < 0.0001 Versus GES-1 or pcDNA-control; ^###p < 0.001, ^####p < 0.0001 versus si-NC or pcDNA-GPX8