Fig. 5.

Evolutionarily constrained region 3′ of PGC1α locus in chromosome 4 with cluster of age-induced hypermethylation. Top associated CpG is highlighted in red. Raw methylation values (0–100%) are shown (tracks with black tick marks) for six samples representing extremes of the age distribution in our sample set (ages shown on the right). Bottom track shows 100 vertebrates Basewise Conservation by PhyloP (phyloP100wayAll): high evolutionary constraints are evident by Phylop constraint scores > 4.88 at 3′ end of the transcript and distal intergenic region, spanned by age-related hypermethylation