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. 2020 Dec 14;39:283. doi: 10.1186/s13046-020-01799-1

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — The characteristics of circRNA_0000392. a The genomic loci of the YAF2 gene and circRNA_0000392. The spliced mature sequence length of circRNA_0000392 is 326 bp. b The expression of circRNA_0000392 and YAF2 mRNA in SW620 and RKO cells treated with or without RNase R was detected by qRT-PCR. c qRT-PCR analysis of circRNA_0000392 and YAF2 mRNA in SW620 cells treated with actinomycin D at the indicated time points. d Fluorescence in situ hybridization (FISH) assays were performed to determine the localization of circRNA_0000392 in SW620 and RKO cells. Scale bar = 50 μm. Data represent the mean ± SD. * P < 0.05, ** P < 0.01