Fig. 5.

CircRNA_0000392 functions as a sponge for miR-193a-5p. a RIP analysis of circRNA_0000392 using anti-AGO2 antibody in SW620 and RKO cells. b The circRNA-miRNA-mRNA interaction based on circRNA_0000392 was demonstrated by prediction and bioinformatics analysis using Cytoscape software. c The top five miRNAs that may be regulated by circRNA_0000392 based on the miRNA prediction and bioinformatics analyses are shown and measured by qRT-PCR after the pull-down assay in RKO cells. d Schematic illustration demonstrating the luciferase reporter vectors containing wild-type (WT) or mutant (MUT) predicted miR-193a-5p binding sites of circRNA_0000392. e The luciferase assay was performed in 293 T cells after cotransfection with miR-193a-5p mimic and the luciferase vector containing wild-type (WT) or mutant (MUT) circRNA_0000392. f Relative expression of miR-193a-5p in 40 pairs of CRC and ANT tissues measured by qRT-PCR. g The correlation between circRNA_0000392 and miR-193a-5p in CRC tissues was analyzed by Spearman correlation coefficients. Data represent the mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001