Unique expression of NOX5 in ALCL cell lines. (A) RT-PCR was performed for NOX5 on cDNAs derived from various lymphoma cell lines: non-Hodgkin (NHL) and Hodgkin (HL) types including anaplastic large-cell lymphoma (ALCL), T cell lymphoma (TCL), Burkitt lymphoma (BL), and large-cell lymphoma (LCL) cells. The bottom shows β-actin as loading control. No cDNA denotes PCR without preceding reverse transcription step. Human tonsil (Tons) cDNA was used as positive control. SR, SR-786 cell line; SUP, SUP-M2 cell line; Karpas, Karpas-299 cell line. (B) Presence of the mRNA segment coding for the activation domain of NOX5 in ALCL cells. Human fetal thymus cDNA was used as positive control. EF, the calcium-binding EF-hand domain. (C) Expression of the NOX5α isoform in ALCL cells. Human spleen cDNA was used as positive and human testis cDNA as negative control.