Table 2. Summary of phenotypes resulting from knockdown of members of the PP2A/4/6 family and their regulators in wildtype N2 C. elegans.
| P. aeruginosa Infection | Heat Stress | UV Irradiation | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| L4 | Day 6 | L4 | Day 6 | L4 | Day 6 | ||||||||
| Average relative LT50 | p-Value | Average relative LT50 | p-Value | Average relative LT50 | p-Value | Average relative LT50 | p-Value | Average relative LT50 | p-Value | Average relative LT50 | p-Value | ||
| PP2A | let-92 | NA | NA | 0.746 | 2.7E-01 | NA | NA | 0.866 | 1.8E-01 | NA | NA | 0.605 | 1.4E-02 |
| paa-1* | NA | NA | 0.897 | 4.7E-01 | NA | NA | 0.802 | 1.5E-01 | NA | NA | 0.767 | 2.5E-02 | |
| pptr-1* | 0.949 | 2.7E-01 | 0.809 | 1.1E-01 | 0.940 | 2.5E-01 | 0.866 | 1.9E-01 | 0.909 | 7.5E-01 | 0.620 | 9.1E-03 | |
| sur-6* | 0.913 | 4.3E-01 | 0.784 | 1.3E-02 | 0.822 | 3.5E-01 | 0.861 | 7.4E-03 | 1.192 | 3.2E-01 | 0.836 | 3.8E-01 | |
| cash-1 | 1.057 | 4.7E-01 | 1.054 | 6.4E-01 | 1.131 | 6.2E-01 | 1.015 | 6.5E-01 | 0.846 | 2.9E-01 | 0.858 | 3.4E-01 | |
| pptr-2 | 0.966 | 3.3E-01 | 0.881 | 4.2E-01 | 0.949 | 5.9E-01 | 0.921 | 3.5E-01 | 0.984 | 9.2E-01 | 0.958 | 7.4E-01 | |
| rsa-1 | 1.014 | 7.1E-01 | 1.150 | 3.9E-01 | 0.937 | 6.1E-01 | 1.111 | 2.4E-01 | 2.664 | 4.5E-01 | 1.005 | 7.4E-01 | |
| F43B10.1* | 0.912 | 1.2E-01 | 0.906 | 6.5E-02 | 0.979 | 8.9E-01 | 1.022 | 6.4E-01 | 0.933 | 6.5E-01 | 0.977 | 6.9E-01 | |
| T22D1.5* | 1.055 | 6.3E-01 | 0.941 | 6.1E-01 | 1.053 | 6.9E-01 | 1.075 | 4.4E-01 | 0.911 | 5.4E-01 | 1.090 | 3.8E-01 | |
| PP4 | pph-4.1* | 0.991 | 8.5E-01 | 0.922 | 2.5E-02 | 0.850 | 1.7E-03 | 1.057 | 4.6E-01 | 0.959 | 8.8E-01 | 0.915 | 5.5E-01 |
| pph-4.2* | 0.898 | 1.5E-01 | 0.526 | 8.6E-03 | 0.922 | 3.7E-01 | 0.954 | 4.4E-01 | 0.756 | 3.7E-01 | 0.869 | 2.9E-01 | |
| smk-1* | 0.887 | 4.7E-04 | 0.570 | 1.7E-10 | 0.985 | 6.3E-01 | 0.904 | 1.3E-01 | 0.878 | 8.2E-02 | 0.707 | 9.8E-04 | |
| ppfr-2 | 0.987 | 7.9E-01 | 0.886 | 4.0E-02 | 1.252 | 4.2E-01 | 1.200 | 2.3E-01 | 1.457 | 3.1E-01 | 1.016 | 9.0E-01 | |
| ppfr-1 | 0.995 | 9.1E-01 | 0.950 | 1.3E-01 | 1.017 | 1.3E-01 | 0.788 | 4.6E-01 | 1.753 | 4.1E-01 | 1.037 | 6.5E-01 | |
| F46C5.6 | 0.972 | 7.9E-01 | 1.083 | 6.0E-01 | 1.270 | 4.2E-01 | 1.269 | 3.3E-02 | 0.925 | 6.0E-01 | 1.164 | 4.3E-01 | |
| PP6 | pph-6 | 1.009 | 7.6E-01 | 0.585 | 1.5E-02 | 1.100 | 1.8E-02 | 0.725 | 1.6E-01 | 0.806 | 3.4E-01 | 0.780 | 8.9E-02 |
| saps-1 | 1.037 | 4.1E-01 | 0.563 | 2.4E-02 | 1.024 | 8.5E-01 | 0.948 | 2.1E-01 | 0.917 | 6.1E-01 | 0.855 | 3.0E-01 | |
| Regulators | Y71H2AM.20* | 1.060 | 2.3E-01 | 0.771 | 1.1E-01 | 1.376 | 1.8E-01 | 0.905 | 1.6E-01 | 1.002 | 1.0E+00 | 0.831 | 2.0E-01 |
| ppfr-4 | 1.098 | 3.8E-02 | 0.843 | 2.4E-01 | 0.989 | 4.5E-01 | 0.929 | 2.4E-01 | 2.106 | 3.8E-01 | 0.674 | 5.5E-02 | |
| ZK688.9 | 0.990 | 8.9E-01 | 0.909 | 4.9E-01 | 1.218 | 3.6E-01 | 1.115 | 1.9E-01 | 0.926 | 7.3E-01 | 1.152 | 1.1E-01 | |
* denotes genes that are required for the increase in Plys-7::GFP expression at Day 6. Average relative LT50 was calculated by dividing the average LT50 of RNAi-treated animals in a given stress assay by the average LT50 of age-matched control animals in that same assay and thus represents the proportional increase or decrease in median lifespan under stressful conditions that are attributable to RNAi inhibition of the gene of interest. The average relative LT50 values corresponding to knockdowns that decreased or increased median lifespan after exposure to stress by more than 10% compared to empty vector control are shaded in orange and blue, respectively. Statistical significance was determined by Student’s t-test, with a threshold of p<0.05. p-values associated with significant susceptibility and resistance are bolded.