Table 3:

Novel genomic methodologies not yet applied to sarcoidosis

Category	Methodology	Advantage
Genetics	Whole-genome sequencing	Observed rather than imputed data, full genome coverage, better copy number variant and rare SNP determination
Epigenetics	Genome-wide methylation array	Quantitative accuracy and reproducibility
Epigenetics	Methylation sequencing (e.g., whole-genome bisulfite sequencing)	High resolution (up to single-base)
Epigenetics	single-cell epigenetics (e.g., single-cell whole-genome bisulfite sequencing)	Localization of epigenetic changes to specific cell types
Transcriptomics	single-cell RNA-sequencing	Localization of transcriptomic changes to specific cell types
Transcriptomics	spatial transcriptomis (e.g., 10x Genomic’s Visium)	Localization of transcriptomic changes to specific physical locations
Transcriptomics	small RNA sequencing (e.g., miRNA-seq)	Amplification of microRNA (miRNA), small interfering RNA (siRNA), and piwi-interacting RNA (piRNA)
Analytics	Meta-analyses	Increased power and diversity of subjects
Analytics	Cross-disease comparison methods (e.g., PRSice and LDPred)	High-resolution scoring with conditioning on covariates or linkage disequilibrium