Figure 1.

(Top) imino region of the 1D ¹H NMR spectrum of CA II in human cells (red) overlaid to the spectrum of the cellular background obtained from cells transfected with empty vector (blue). (Bottom) 1D ¹H NMR spectrum of CA II in aqueous buffer (black). Residues for which the unambiguous assignment has been reported previously are labeled with the corresponding residue number and atom type.^40,41 H94, H96, and H119 coordinate the zinc ion in the active site. Signals arising from unassigned protons are labeled with an asterisk.