Fig 1. Exogenous auxin treatment enhances rice tolerance to RDV infection via down-regulating OsIAA10.

(A) IAA content is higher in RDV-infected plants. ZH11-Mock, uninfected ZH11 plants, ZH11-RDV, RDV-infected ZH11. (B) Increased expression of some auxin biosynthesis genes in RDV-infected rice. ZH11-Mock, uninfected ZH11 plants, ZH11-RDV, RDV-infected ZH11. (C) Phenotypes of RDV-infected ZH11 rice plants pretreated with H₂O, IAA or NAA, respectively. Photos were taken at four-week-post-inoculation (wpi). Scale bars, 10 cm (upper panel) and 1 cm (lower panel). (D) Schematic representation of plant height for the plants in (C). The average (±SD) values were obtained from three biological repeats. Different letters indicate significant difference (p< 0.05) based on the Tukey-Kramer HSD test. (E) Western blots showing the accumulation of RDV proteins in the corresponding rice lines shown in (C). Actin was used as a loading control. (F) Western blots showing the accumulation of OsIAA10 protein after auxin treatment and in the ZH11 (Mock). Actin was used as a loading control. (G) RDV-infected WT (ZH11) and osiaa10 KO rice plants. Photos were taken at 4 weeks after RDV-inoculation. The sizes of white specks on the leaves represent the degree of disease symptoms. Scale bars, 10 cm (upper panel) and 1 cm (lower panel). (H) Schematic representation of plant height for the plants in (G). The average (±SD) values were obtained from three biological repeats. Different letters indicate significant difference (p< 0.05) based on the Tukey-Kramer HSD test. (I) qRT-PCR showing the accumulation of RDV genomic RNAs in the corresponding rice lines shown in (G). (J) Western blots showing the accumulation of RDV proteins in the corresponding rice lines in (G). Actin was used as a loading control.