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. 2020 Dec 2;18(12):e3000941. doi: 10.1371/journal.pbio.3000941

Fig 5. Improved recovery of cardiac function in YAP/TAZ-deficient mice post-MI.

Fig 5

(A–E) Echocardiographic measurements of ventricular functional parameters at baseline and 4-week post-MI from control and YAP/TAZ-dKO mice: (A) LVEF; (B) FS; (C) LVESV; (D) FAC; (E) Representative echocardiographic M-mode images of LV. Data are shown as mean ± SEM (n = 4 to 7 per group). (F–H) Flow cytometry analysis of pro-inflammatory (iNOS+/F4/80+) and reparative (CD206+/F4/80+) macrophages from the 2 and 6 days post-MI hearts: (F) percentage of pro-inflammatory (F4/80+iNOS+) and reparative (F4/80+CD206+) macrophages. Underlying raw data can be found in S1 Data and S1, S2, S3, S4, S5, S6, S7, S8, S9, S10, S11, S12, S13, S14, S15, S16, S17, S18, S19, S20, S21 and S22 FCS files. (G) qRT-PCR for pro-inflammatory cytokines IL6, IL1β, and Nos2 in isolated iNOS+/F4/80+ macrophages from the 2 days post-MI hearts. (H) qRT-PCR for anti-inflammatory markers Arg1, Ym1, and Fizz1 in isolated CD206+/F4/80+ macrophages from the 6 days post-MI hearts. (I) qRT-PCR for pro-inflammatory marker genes, IL6, IL1β, TNFα, Nos2, Ccl2, Ccr7, and Rantes using RNA isolated from control and YAP/TAZ-dKO hearts (LV) at 3 days post-MI. n = 4 in each group. (J) qRT-PCR for anti-inflammatory marker genes, Ym1, Egr2, Arg1, Fizz1, Cd206, IL10, and TGFβ using RNA isolated from control and YAP/TAZ-dKO hearts (LV) at 7 days post-MI. n = 3 to 4 in each group. Gene expression results were normalized to Gapdh, and results are represented as fold change. For numerical raw data, please see S1 Data. Arg1, Arginase-I; Ccl2, C–C motif chemokine ligand 2; Ccr7, C-C chemokine receptor type 7; Cd206, cluster of differentiation 206; Egr2, early growth response 2; FAC, fractional area change; Fizz1, found in inflammatory zone 1; FS, fractional shortening; Gapdh, glyceraldehyde-3-phosphate dehydrogenase; IL, interleukin; LVEF, left ventricular ejection fraction; LVESV, left ventricular end-systolic volume; MI, myocardial infarction; Nos2, nitric oxide synthase 2; NS, non-significant; qRT-PCR, real-time quantitative reverse transcription PCR; TAZ, transcriptional coactivator with PDZ-binding motif; TGFβ, transforming growth factor beta; TNFα, tumor necrosis factor alpha; YAP, yes-associated protein; YAP/TAZ-dKO, YAP/TAZ double knockout.