Figure S2.

GRASP55 and 65 can be degraded in nocodazole-treated cells. (A) Scheme of the experiment. RC55, RC65, and RC65+55 cells were treated with doxycycline (dox) for 6 h. IAA was then added to degrade GRASPs and nocodazole (noc) to depolymerize microtubules and to disperse Golgi stacks. (B) 2 h later, the cells were fixed and immunostained for Golgin-84 (red) and mAID (green) and labeled for DNA (blue). Scale bar, 10 µm. (C) Quantification of the mAID fluorescence signal on the Golgi (labeled by Golgin-84). n ≥ 3 independent experiments with >50 cells analyzed per experiment and condition. *** P < 0.001; **** P < 0.0001. Error bars represent mean ± SD. Scale bar, 10 µm.