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. Author manuscript; available in PMC: 2020 Dec 19.
Published in final edited form as: Science. 2020 Jun 19;368(6497):1386–1392. doi: 10.1126/science.aaz4427

Fig. 1.

Fig. 1.

Nuclear condensates in human tissue and in vitro. (A) Model illustrating potential behaviors of small molecules in nuclear condensates. (B–C) Immunofluorescence of scaffold proteins of various nuclear condensates in tissue biopsies from benign and malignant human breast (B), and benign and malignant colon tissue (C), in nuclei stained with Hoechst, imaged at 100× on a fluorescent confocal microscope (see also Figures S1, S2). (D) Schematic of in vitro droplet formation assay to measure small molecule partitioning into nuclear condensates. (E) In vitro droplet assay showing the behavior of fluorescein dye in the presence of six protein condensates formed in 125mM NaCl and 10% PEG, with 10μM protein and 5μM fluorescein, imaged at 150× on a confocal fluorescent microscope (see also Figures S3S6). Quantification of enrichment of the drug is shown to the right, error bars represent SEM.