Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Biological sample (Mus musculus) | C57BL/6 primary alveolar epithelial cells | Cell Biologics | Cat#: C57-6053 | |
| Biological sample (Mus musculus) | C57BL/6 primary lung microvascular endothelial cells | Cell Biologics | Cat#: C57-6011 | |
| Other | Epithelial cell culture medium with kit | Cell Biologics | Cat#: M6621 | |
| Other | Endothelial cell culture medium with kit | Cell Biologics | Cat#: M1168 | |
| Strain, strain background (Mus musculus, C57BL6) | Tg(act-EGFP) 131Osb/LeySopJ | Jackson Laboratory | Cat#: 006567; RRID:IMSR_JAX:006567 | Female, 6–8 weeks old |
| Peptide, recombinant protein | Murine recombinant M-CSF | ThermoFisherScientific | Cat#: PMC2044 | |
| Commercial assay or kit | Superscript IV First Strand Synthesis System | Thermo Fisher Scientific | Cat#: 18091050 | |
| Commercial assay or kit | Sybr Green PCR Master Mix | Thermo Fisher Scientific | Cat#: 4334973 | |
| Antibody | Anti-mouse proSPC (Rabbit polyclonal) | Abcam | Cat#: ab40879; RRID:AB_777473 | IF(1:100) |
| Antibody | Anti-mouse Podoplanin-488 (Syrian Hamster monoclonal) | Thermo Fisher Scientific | Cat#: 53-5381-82; RRID:AB_1106990 | IF(1:100) |
| Antibody | Anti-mouse CD45-647 (Rat monoclonal) | BioLegend | Cat#: 103124; RRID:AB_493533 | IF (1:100) |
| Commercial assay or kit | Lung dissociation kit - mouse | Miltenyi Biotec | Cat#: 130-095-927 | |
| Peptide, recombinant protein | Fibronectin from human plasma | Sigma-Aldrich | Cat#: F1056 | |
| Peptide recombinant protein | Native Collagen, Bovine dermis | AteloCell | Cat#: IAC-50 | 5 mg/ml |
| Software, algorithm | FIJI | RRID:SCR_002285 | ||
| Software, algorithm | MATLAB | RRID:SCR_001622 |