Extended Data Figure 6. RTT patient mutations disrupt MeCP2 condensate formation.

a. Droplet areas for experiments in Fig. 3b. Fields per condition n=15.

b. Droplet areas for experiments in Fig. 3d. Fields per condition n=15.

c. Droplet areas for experiments in Fig. 3f. Fields per condition n=15.

d. Droplet experiments examining effects of RTT patient missense mutations that disrupt IDR-2 on MeCP2 droplet formation. MeCP2-GFP WT and RTT IDR-2 mutants (P225R and P322L) at indicated concentrations were mixed with 20 nM methylated DNA in droplet formation buffers with 100 mM NaCl.

e. MeCP2-GFP condensed fraction as a function of MeCP2-GFP concentration for experiments in Extended Data Fig. 6d. Mean±SD. Fields per condition n=15.

f. Droplet areas for experiments in Extended Data Fig. 6d. Fields per condition n=15.