a. Droplet experiments examining ability of MeCP2 WT and R306C mutant condensates to enrich TBLR1-CTD. MeCP2-GFP WT or R306C mutant at 6 μM was mixed with TBLR1-CTD-mCherry at 10 μM in droplet formation buffers with 125 mM NaCl and 10% PEG-8000.
b. TBLR1-CTD-mCherry partition ratios in MeCP2-GFP WT and R306C mutant droplets for experiments in Extended Data Fig. 7a. Fields per condition n=15.
c. Droplet experiments examining ability of MeCP2 WT and R306C mutant condensates to enrich TBLR1-CTD. MeCP2-GFP WT or R306C mutant at 10 μM was mixed with TBLR1-CTD-mCherry at 4 μM in droplet formation buffers with 125 mM NaCl.
d. TBLR1-CTD-mCherry partition ratios in MeCP2-GFP WT and R306C mutant droplets for experiments in Extended Data Fig. 7c. Fields per condition n=12.