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. Author manuscript; available in PMC: 2021 Apr 1.
Published in final edited form as: Nature. 2020 Jul 22;586(7829):440–444. doi: 10.1038/s41586-020-2574-4

Extended Data Figure 7. TBLR1 partitioning into MeCP2 droplets is disrupted by RTT mutation R306C.

Extended Data Figure 7.

a. Droplet experiments examining ability of MeCP2 WT and R306C mutant condensates to enrich TBLR1-CTD. MeCP2-GFP WT or R306C mutant at 6 μM was mixed with TBLR1-CTD-mCherry at 10 μM in droplet formation buffers with 125 mM NaCl and 10% PEG-8000.

b. TBLR1-CTD-mCherry partition ratios in MeCP2-GFP WT and R306C mutant droplets for experiments in Extended Data Fig. 7a. Fields per condition n=15.

c. Droplet experiments examining ability of MeCP2 WT and R306C mutant condensates to enrich TBLR1-CTD. MeCP2-GFP WT or R306C mutant at 10 μM was mixed with TBLR1-CTD-mCherry at 4 μM in droplet formation buffers with 125 mM NaCl.

d. TBLR1-CTD-mCherry partition ratios in MeCP2-GFP WT and R306C mutant droplets for experiments in Extended Data Fig. 7c. Fields per condition n=12.