a. Schematic of MeCP2-GFP chimeric mouse generation using endogenous-tagged MeCP2-GFP mESCs. Endogenous-tagged MeCP2-GFP mESCs derived from V6.5 background were injected into embryos from CD1-IGS mice and multiple embryos were implanted into pseudo-pregnant female mice. Chimeric pups were distinguished from non-chimeric littermates by agouti coat color. MeCP2-GFP tagged adult chimeric mice were used for experiments.
b. Schematic of strategy used to generate endogenous-tagged MeCP2-GFP mESCs.
c. PCR genotyping of endogenous-tagged MeCP2-GFP mESCs. For gel source data, see Supplementary Figure 1.
d. MECP2 expression values in transcripts per million (TPM) measured by RNA-seq for various human tissues surveyed by GTEx. Tissues are ordered based on expression level. TPM values greater than 1 are considered to be expressed.