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. Author manuscript; available in PMC: 2021 Apr 1.
Published in final edited form as: Nature. 2020 Jul 22;586(7829):440–444. doi: 10.1038/s41586-020-2574-4

Extended Data Figure 2. Generation of endogenous-tagged MeCP2-GFP chimeric mice.

Extended Data Figure 2.

a. Schematic of MeCP2-GFP chimeric mouse generation using endogenous-tagged MeCP2-GFP mESCs. Endogenous-tagged MeCP2-GFP mESCs derived from V6.5 background were injected into embryos from CD1-IGS mice and multiple embryos were implanted into pseudo-pregnant female mice. Chimeric pups were distinguished from non-chimeric littermates by agouti coat color. MeCP2-GFP tagged adult chimeric mice were used for experiments.

b. Schematic of strategy used to generate endogenous-tagged MeCP2-GFP mESCs.

c. PCR genotyping of endogenous-tagged MeCP2-GFP mESCs. For gel source data, see Supplementary Figure 1.

d. MECP2 expression values in transcripts per million (TPM) measured by RNA-seq for various human tissues surveyed by GTEx. Tissues are ordered based on expression level. TPM values greater than 1 are considered to be expressed.