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. Author manuscript; available in PMC: 2021 Apr 1.
Published in final edited form as: Nature. 2020 Jul 22;586(7829):440–444. doi: 10.1038/s41586-020-2574-4

Extended Data Figure 5. MeCP2 condensate partitioning of BRD4 domains.

Extended Data Figure 5.

a. Droplet experiments examining ability of MeCP2 condensates to preferentially incorporate and concentrate HP1α compared to BRD4 domains in the presence of naked DNA. BRD4-IDR, bromodomain 1 (BD1), and extra-terminal (ET) domain were examined. MeCP2-GFP at 7.5 μM was mixed with either HP1α-mCherry, BRD4-IDR-mCherry, BRD4-BD1-mCherry, BRD4-ET-mCherry, or mCherry each at 7.5 μM and 160 nM methylated DNA in droplet formation buffers with 150 mM NaCl and 10% PEG-8000.

b. mCherry partition ratios in MeCP2-GFP droplets for experiments in Extended Data Fig. 5a. Fields per condition n=15.