TABLE 4.
Necropsy and Histology Results
| Subject | Cell concentration | Cell infusion location | Saline infusion location | Gross pathology | Histology and GFP |
|---|---|---|---|---|---|
| Canine 1 | 2 × 106 cells/20 mL | L ICA | R ICA | No significant lesions observed | No significant lesions observed. |
| Canine 2 | 1 × 107cells/20 mL | L ICA | R ICA | No significant lesions observed | Brain edema, meningeal, moderate, multifocal, acute. No difference observed between hemispheres. |
| No significant lesions observed in peripheral organs. | |||||
| Canine 3 | 2 × 107cells/20 mL | L ICA | R ICA | No significant lesions observed | No significant lesions observed. |
| Canine 4 | 1 × 108cells/20 mL | R ICA | L ICA | No significant lesions observed | No significant lesions observed. |
| Canine 5 | 1 × 108 cells/20 mL | L PCA | R PCA | No significant lesions observed | No evidence of stroke or vascular obstruction was observed in the brain. |
| No significant lesions observed in peripheral organs. | |||||
| Canine 6 | 1 × 108 cells/10 mL | L PCA | R PCA | Perivascular hemorrhage around distal BA | Hemorrhage, leptomeningeal, focally extensive and moderate, multifocal, acute. No evidence of stroke or vascular obstruction was observed in the brain. |
| No significant lesions observed in peripheral organs. | |||||
| Canine 7 | Positive stroke control | R PCA particles | no infusion | Cisterna magna SAH; peri-medullary hemorrhage | Hemorrhage, leptomeningeal, regional (caudal brain stem), moderate, multifocal coalescing, acute. |
| Intravascular foreign bodies, bilateral small arterioles – moderate number, acute. No evidence of stroke other than particle obstruction. | |||||
| Canine 8 | Positive stroke control | R ICA particles | no infusion | No significant lesions observed | Intracerebral intravascular beads, marked, diffuse, acute, also in the eye (choroid and optic nerve), and meninges. No morphologic evidence of ischemia was observed in the brain or eye. |
| Canine 9 | 1 × 108 cells/10 mL | R ICA | L ICA | No significant lesions observed | No morphologic indications of ischemia in the brain or other tissues are observed |
| Canine 10 | 1 × 108 cells/10 mL | L ICA | R ICA | No significant lesions observed | No morphologic indications of ischemia in the brain or other tissues are observed. Clump of large mononuclear round cells seen in a large meningeal artery. |
Incidental findings (eg, hepatocellular lipidosis) that were independently determined to be unrelated are available in supplemental data reports. BA = basilar artery.