Figure 5.
Topical ocular treatment of WT mice with AKB-9778 increases SC filtration area. (A) Four-week-old C57Bl6 (WT) or Tie2+/− mice were treated twice daily with eyedrops containing AKB-9778 or vehicle for 4 weeks. SC area was analyzed by PECAM-1 immunofluorescence staining of cornea whole mounts. The 20× fields shown represent an area of 146,000 µm2. Images were captured as Z stacks with a step size of 0.89 µm and a pinhole of 1 airy unit, and are shown as maximum intensity projections. (B) For quantification SC area was measured in sixteen 20× images per condition for 8 WT and 7 Tie2+/− mice; **P ≤ 0.001, ****P ≤ 0.0001.