TABLE 1.
Statistical results for unpaired t, Kruskal-Wallis, one-way and two-way ANOVA test analyses.
| Figure number | Test used | n | Data reported | P-value | F(DFn, DFd) | t | K | DF |
| Figure 1D | Two way-ANOVA | n: 3–4 | Mean ± SEM | Interaction: P = 0.2716 | Interaction F(5, 30) = 1.340 | Interaction: 5 | ||
| Column factor: P = 0.0514 | Column factor: F(1, 30) = 4.116 | Column factor: 1 | ||||||
| Figure 2A | Two way-ANOVA | n: 7–26 | Mean ± SEM | Interaction: P = 0.0001 | Interaction F(8, 188) = 5.983 | Interaction: 8 | ||
| Column factor: P = 0.0001 | Column factor: F(2, 188) = 91.68 | Column factor: 2 | ||||||
| Figure 2B | Two way-ANOVA | n: 7–26 | Mean ± SEM | Interaction: P = 0.0001 | Interaction F(4, 144) = 9.825 | Interaction: 4 | ||
| Column factor: P = 0.0182 | Column factor: F(1, 144) = 5.711 | Column factor: 1 | ||||||
| Figure 2C | Unpaired t test | n: 23 | Mean ± SEM | P = 0.0001 | t = 4.620 | |||
| Figure 2D | Unpaired t-test | n:7 | Mean ± SEM | P = 0.7791 | t = 22.00 | |||
| Figure 3A | Kruskal-Wallis test | n:4–7 | Mean ± SEM | P = 0.0002 | K = 33.47 | |||
| Figure 3B | Kruskal-Wallis test | n:4–7 | Mean ± SEM | P = 0.0001 | k = 41.40 | |||
| Figure 3C | Two way-ANOVA | n:4–7 | Mean ± SEM | Interaction: P = 0.0001 | Interaction F(14, 112) = 4.220 | Interaction: 14 | ||
| Column factor: P = 0.0001 | Column factor: F(2, 112) = 16.18 | Column factor: 2 | ||||||
| Figure 3D | Two way-ANOVA | n:4–7 | Mean ± SEM | Interaction: P = 0.0001 | Interaction F(14, 104) = 4.363 | Interaction: 14 | ||
| Column factor: P = 0.0001 | Column factor: F(2, 104) = 16.48 | Column factor: 2 | ||||||
| Figure 3E | Two way-ANOVA | n:4–7 | Mean ± SEM | Interaction: P = 0.0001 | Interaction F(14, 104) = 3.864 | Interaction: 14 | ||
| Column factor: P = 0.0014 | Column factor: F(2, 104) = 7.030 | Column factor: 2 | ||||||
| Figure 3F | Two way-ANOVA | n:4–7 | Mean ± SEM | Interaction: P = 0.0009 | Interaction F(14, 96) = 2.942 | Interaction: 14 | ||
| Column factor: P = 0.2162 | Column factor: F(2, 96) = 1.556 | Column factor: 2 | ||||||
| Figure 3G | Two way-ANOVA | n:4–7 | Mean ± SEM | Interaction: P = 0.0839 | Interaction F(14, 93) = 1.636 | Interaction: 14 | ||
| Column factor: P = 0.2253 | Column factor: F(2, 93) = 1.514 | Column factor: 2 | ||||||
| Figure 4A | Kruskal-Wallis test | n: 4–6 | Mean ± SEM | P = 0.5599 | k = 1.559 | |||
| Figure 4B | Kruskal-Wallis test | n:4–6 | Mean ± SEM | P = 0.5924 | K = 1.140 | |||
| Figure 4C | Kruskal-Wallis test | n: 4–6 | Mean ± SEM | P = 0.0449 | k = 5.843 | |||
| Figure 4D | Kruskal-Wallis test | n:4–6 | Mean ± SEM | P = 0.0101 | K = 7.896 | |||
| Figure 4E | Kruskal-Wallis test | n: 4–6 | Mean ± SEM | P = 0.0054 | k = 8.643 | |||
| Figure 5a | Unpaired test | n:4–6 | Mean ± SEM | P = 0.0095 | t = 0.0 | |||
| Figure 5B | Unpaired test | n: 4–6 | Mean ± SEM | P = 0.1143 | t = 6.000 | |||
| Figure 5C | Two way-ANOVA | n: 4–6 | Mean ± SEM | Interaction: P = 0.0625 | Interaction F(4, 40) = 2.439 | Interaction: 4 | ||
| Column factor: P = 0.0173 | Column factor: F(1, 40) = 6.169 | Column factor: 1 | ||||||
| Figure 5D | Unpaired test | n: 4–6 | Mean ± SEM | P = 0.8252 | t = 11.00 | |||
| Figure 5E | Unpaired test | n: 4–6 | Mean ± SEM | P = 0.3476 | t = 10.00 | |||
| Figure 5F | Unpaired test | n: 4–6 | Mean ± SEM | P = 0.3448 | t = 9.00 |
n, number of animals used in this study; SEM, standard error of the mean; F, F statistic; DFn, numerator degrees of freedom; DFd, denominator degrees of freedom; K, Kruskal-Wallis statistic; DF, degrees of freedom, t, unpaired t-test statistic. D’Agostino and Pearson omnibus normality test was applied to evaluate the samples normality. Two groups comparison was performed using a unpaired t-test. The comparison between more of one non-parametric groups was performed using a Kruskal-Wallis test, while comparisons between parametric samples were made through One way-ANOVA. Comparisons between two groups and two variables were made by applying a Two way-ANOVA. No animals were excluded from the statistical analysis.