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RNA and protein expression in parent WRO and WRO-LvR cells were evaluated with or without treatment with 2 μM KU757 for 48 h. Analysis of glycolytic genes (Figure 5A) and proteins (Figure 5B) by RT-PCR and western blot indicate that glycolytic genes and proteins are upregulated in WRO-LvR cells compared to parent WRO cells. Treatment of WRO-LvR cells with KU757 results in downregulation of glycolytic enzymes.
