FIGURE 3.

Phylogenetic tree of the bacteria based on the SadA sequence. The SadA sequence from S. pseudintermedius ED99 (WP_014612792) was used as a reference to identify homologs within the domain of Bacteria using BLASTP. All best hits were used to construct a phylogenetic tree, using the Randomized Axelerated maximum-likelihood (RaxML) method (version8.2.9) (Sievers et al., 2011). SadA was widespread among bacteria and could be found in at least seven phyla. Based on the SadA sequences, phyla did not cluster perfectly, but the subtrees often consisted of one phylum. The list of bacterial species is shown in Supplementary Table 1.