FIGURE 4.

Phylogenetic tree of the SadA sequences within the Firmicutes. The SadA sequence from S. pseudintermedius ED99 (WP_014612792) was used as a reference to identify homologs within the Firmicutes phylum using BLASTP. All best hits were used to construct a phylogenetic tree, using the Randomized Axelerated maximum-likelihood (RaxML) method (version8.2.9) (Sievers et al., 2011). SadA was found in many genera of Firmicutes. Based on the SadA sequences, genera were not clustered perfectly, but the subtrees often consisted of one genus. The list of bacterial species is shown in Supplementary Table 2.