Fig. 5 |. Interface residues on Rubisco are required for yeast two-hybrid interactions between EPYC1 and Rubisco, and for pyrenoid matrix formation in vivo.

a, The importance of Rubisco small subunit residues for interaction with EPYC1 was tested by mutagenesis in a yeast two-hybrid experiment. b, The Rubisco small subunit-less mutant T60 (Δrbcs) was transformed with wild-type, D23A/E24A or M87D/V94D Rubisco small subunits. Serial 1:10 dilutions of cell cultures were spotted on TP minimal medium and grown in air or 3% CO₂. c-d, Representative electron micrographs of whole cells (c) and corresponding pyrenoids (d) of the strains expressing wild-type, D23A/E24A, and M87D/V94D Rubisco small subunit. Dashes in panel c indicate regions shown in panel d. Scale bars = 500 nm. At least 25 cells were imaged for each strain; additional representative images are shown in Extended Data Fig. 10c.