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. Author manuscript; available in PMC: 2021 May 23.
Published in final edited form as: Nat Plants. 2020 Nov 23;6(12):1480–1490. doi: 10.1038/s41477-020-00811-y

Extended Data Fig. 2. Single-particle cryo-EM data collection and image processing procedure.

Extended Data Fig. 2

a-c, Representative micrographs of the apo Rubisco sample (a), the Rubisco-EPYC149-72 complex (b) and the Rubisco-EPYC1106-135 complex (c). Scale bars = 100 nm. d-f, Representative 2D class averages of the apo Rubisco sample (d), the Rubisco-EPYC149-72 complexes (e) and the Rubisco-EPYC1106-135 complexes (f). g-i, Overview of the workflow for single-particle data processing for the apo Rubisco sample (g), the Rubisco-EPYC149-72 sample (h) and the Rubisco-EPYC1106-135 sample (i). j-l, Local resolution estimation of the final refined apo Rubisco map (j), the final refined Rubisco-EPYC149-72 complex map (k) and the final refined Rubisco-EPYC1106-135 complex map (l).