Table 1.
The primer sequences for RT-PCR.
| Genes | Forward (5′–3′) | Reverse (5′–3′) |
|---|---|---|
| TNF-α (mouse) | CGTCAGCCGATTTGCTATCT | CGGACTCCGCAAAGTCTAAG |
| IL-1β (mouse) | TCAGGCAGGCAGTATCACTC | AGCTCATATGGGTCCGACAG |
| IL-18 (mouse) | GACAGCCTGTGTTCGAGGATATG | TGTTCTTACAGGAGAGGGTAGAC |
| TLR4 (rat) | TACAGTTCGTCATGCTTTCTC | ATTAGGAAGTACCTCTATGCAG |
| TLR4 (mouse) | AGCTTCTCCAATTTTTCAGAACTTC | TGAGAGGTGGTGTAAGCCATGC |
| MyD88 (rat) | AGGACAAACGAAGGAACTTTT | GCCGATAGTCTGTCTGTTCTAGT |
| MyD88 (mouse) | ACCTGTGTCTGGTCCATTGCCA | GCTGAGTGCAAACTTGGTCTGG |
| (ZO)-1 (rat) | GCTCACCAGGGTCAAAATGT | GGCTTAAAGCTGGCAGTGTC |
| (ZO)-1 (mouse) | GTTGGTACGGTGCCCTGAAAGA | GCTGACAGGTAGGACAGACGAT |
| Occludin (rat) | TTACGGCTATGGAGGGTACAC | GACGCTGGTAACAAAGATCAC |
| Occludin (mouse) | TGGCAAGCGATCATACCCAGAG | CTGCCTGAAGTCATCCACACTC |
| β-Actin (rat) | GTCGTACCACTGGCATTGTG | CTCTCAGCTGTGTGTGTGAA |
| β-Actin (mouse) | CTCTCAGCTGTGTGTGTGAA | TGCTGGAAGGTGGACAGTGAGG |