Figure 2.

Methyltransferase activity of all the variants tested in this study. The activity was measured using an end-point assay quantifying the concentration of s-adenosylhomocysteine after 2 h at 37 °C in Tris–HCl 50 mM, MgCl₂ 30 mM, 25 mM ATP as described in the Methods. Activities were measured in triplicates or duplicates (K41D, K41E, D138E, E207H) in at least 2 independent experiments. Data represent median values normalised to the WT activity. Error bars represent interquartile range of the activity distributions. All values and standard deviations are reported in the Supplementary Table 1.