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. Author manuscript; available in PMC: 2022 Jan 1.
Published in final edited form as: Surgery. 2020 Jun 29;169(1):155–161. doi: 10.1016/j.surg.2020.04.034

Figure 3. SST knockdown increases cell viability and growth in BON cells.

Figure 3

(a) Immunofluorescence image of SST-knockdown and control BON cells. Knockdown was accomplished by reverse-transfection with two siRNA against SST and a scrambled siRNA as a control. (b) Cell viability of SST-knockdown and control cells at day 3 and 6 post-transfection. Cell viability was determined using alamarBlue assay. (c) Immunofluorescence image of stable SST-knockdown and control cells. Knockdown was accomplished by infection with lentivirus encoding two shRNAs against SST and a scrambled shRNA as a control. (d) Cell count of stable SST-knockdown and control cells, determined by manual counting using a hemocytometer every two days. Data represent mean ± SEM from triplicates.

Abbreviations: short interfering RNA (siRNA), short hairpin RNA (shRNA), somatostatin (SST)