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. 2020 Dec 14;10:21934. doi: 10.1038/s41598-020-78667-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Accumulation of spherulous cells with electron-dense spherules at the surface 1 day post-treatment. Microscopic section (100× magnification) of 1d-samples showing that, compared to control (a), densely-stained spherulous cells accumulated at the wound (arrow) in grazed (b) and mechanical damage (c) groups. (d) TEM-image at the wound confirming that densely-stained spherulous cells correspond with spherulous cells containing electron-dense spherules (ds), with numerous electron-dense granules (g). (e) The surface of control group contained spherulous cells with electron-lucent spherules (arrowhead). (f) The surface of wounding group contained spherulous cells with electron-dense spherules (arrow).