Fig. 4.

Negative viral RNA content in cells with or without inhibitors infected with WT and G2A live viruses. 293S cells without NH₄Cl were infected with live WT, G2A virus and progeny non-myristoylated viruses, and cells with NH₄Cl were infected with live WT virus. Cells with or without NH₄Cl were harvested at indicated time points. Negative viral RNA content was then measured. The RNA levels of viruses were normalized to GAPDH. Data presented as mean ± SD of three independent experiments. Live-WT: live myristoylation virus; Live-G2A: live myristoylation-deficient viruses. Live-WT + 2HMA: Progeny viruses without myristoylation, Live-WT + NH₄Cl: live myristoylation virus infected cells with inhibitor NH₄Cl, ***P < 0.001.